后期酰基转移酶编码基因lpxL对禽致病性大肠杆菌菌株E058致病性的影响

doi:10.11843/j.issn.0366-6964.2016.09.018

Abstract

Abstract:

The contribution of the gene lpxL to cell membrane structures and bionomics of avian pathogenic Escherichia coli (APEC) was studied to reveal the pathogenic mechanism of APEC to avian.An isogenic lpxL mutant was constructed from APEC O2 strain E058,and then compared its structure，biological characteristics，and pathogenicity with E058.The results showed that compared with E058，the lpxL mutant had a smoother surface，slower growth rate and reduction of endotoxin.Nitric oxide production reduction and cytokine gene transcription downregulation also were observed in HD11 treated with mutant-derived LPS relative to that in cells treated with E058-derived LPS at different times.The histopathological lesions in visceral organs of birds challenged with the wild-type strain were more severe than in birds infected with the mutant.These results indicated that the lpxL gene is important for the pathogenicity and biological activity of APEC strain E058.

CLC Number:

S852.612

XU Hui-qing，GAO Lu，MU Xiao-hui，GAO Qing-qing，LING Jie-lu，GAO Song. Effect of the lpxL Lipid A Biosynthesis Pathway Gene on Pathogenicity of Avian Pathogenic Escherichia coli Strain E058 to Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1888-1896.

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Effect of the lpxL Lipid A Biosynthesis Pathway Gene on Pathogenicity of Avian Pathogenic Escherichia coli Strain E058 to Chicken

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